Probing Amyloid Aggregation and Morphology In Situ by Multiparameter Imaging and Super-Resolution Fluorescence Microscopy

Chapter Outline Introduction 105 Multi-Parameter Microscopy of Protein Aggregation Kinetics 106 Fusion Protein Labeling of Amyloidogenic Proteins 106 Principle of Multi-Parameter Imaging Techniques 107 Fluorescence Anisotropy Imaging 107 Principle of HomoFRET in the Study of Protein SelfAssembly Reactions 107 HomoFRET Imaging for the Study of α-synuclein Aggregation 108 Fluorescence Lifetime Imaging of Protein Aggregation 108 The Fluorescence Lifetime of Peptide Labels Reports on Amyloid Aggregation 108 In Vivo FLIM Imaging of Protein Aggregation 110 Super-Resolution Imaging of Amyloid Aggregation 111 Single-Molecule-Based Localization Microscopy for Direct Visualization of Amyloid Morphology 111 Fluorophores for Localization Microscopy 112 Realization of Direct Stochastic Optical Reconstruction Microscopy Experiments 113 In Situ Imaging of Abeta 40 and Abeta 42 Self Assembly Reactions 113 Super-Resolution Imaging of Human Lysozyme 115 Conclusion 115 Acknowledgments 117